Type of project: MSc internship or thesis
Duration: 6 months
Start date: July 2026
Location: Wetsus, European Centre of Excellence for Sustainable Water Technology, Leeuwarden, the Netherlands
Background
Research has demonstrated the important role of the human gut microbiome in regulating human health and susceptibility to disease. Moreover, there is ample research evidence demonstrating the impact of different dietary interventions on the gut microbiome. Even though drinking water is an integral part of a healthy diet, its impact on the human gut microbiome is poorly understood mainly because of the lack of appropriate controls and confounding factors in-vivo. These challenges necessitate an in-vitro approach that can simulate a drinking water-dependent response. While there are many protocols explaining how to simulate the gut microbiome environment outside the human body, they do not incorporate drinking water. In general, the overall impact of drinking water on human health via the gut microbiome has been overlooked.
Research goals
The main goal of this project is to simulate the gut microbiome in-vitro in the presence of different drinking water types and modifications of the chosen culture medium. Then, the community composition and function of the microbiome will be monitored with molecular and analytical techniques to
(1)Trace drinking water bacteria in the gut microbiome and
(2)Identify changes in the pH, gas composition and specific metabolite concentrations
The aim is to capture a drinking-water dependent response by comparing with a reference culture that lacks drinking water. These results may reveal a link between drinking water types and health mediated by the gut microbiome
Your responsibilities
-Process human fecal samples in an anaerobic chamber and carry out fermentation experiments in batch
-Perform molecular and analytical techniques such as flow cytometry, DNA / RNA extraction and qPCR
-Record and interpret experimental data
-Write an MSc thesis / article manuscript if required
-Present data according to conventional scientific standards
Our requirements
-EU citizen or international student registered at a Dutch university
-Enrolled in Microbiology, Biomedical sciences, food technology MSc program or a related field
-Overall interest in microbiology and/or molecular biology
-Previous experience in analytical and molecular biology techniques such as flow cytometry, DNA extraction and PCR is a plus.
-Good verbal and written communication skills in English
-Reliable, organized and able to work in large teams
Your benefits
-Gain hands-on laboratory experience in various molecular & analytical techniques
-Receive close guidance via weekly or biweekly meetings
-Experience the multidisciplinary and collaborative research environment at Wetsus
-Join a large pool of thesis/internship students in an international working environment
-Allowance of 425 EUR per month. Grant holding students (i.e. Erasmus or other) receiving financial support of less than 425 € per month can apply for a top-up supplement to the maximum allowance.
How to apply:
Interested students are invited to send a motivation letter (max. 1 page) and a CV (max. 2 pages) to Adel Bou Alia (Adel.BouAlia@Wetsus.nl) or via the application form below. In the email, please indicate anticipated starting month and how long you are available.